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KMID : 0380020080230010096
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 1 p.96 ~ p.100
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Antioxidant activity analysis of Catechin compounds in Korean green tea using HPLC On-line Antioxidant screening system
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Lee Kwang-Jin
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Abstract
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In this work, we describes analysis of the antioxidant potential of Korean green tea phenolics using an high-performance liquid chromatography (HPLC) on-line antioxidant screening method. In conjunction with the analysis of their 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) radical scavenging ability, the extraction of catechine compounds from Korean green tea were performed by various temperature and time. The optimum operating conditions were experimentally determined to analyze the catechine compounds in the pretreatment extracts. From the results, the extraction temperature , time 3 min was selected as an optimal antioxidant activity condition. The analysis by column was performed, the flow rate of mobile phase and UV wavelength was fixed at 1.0 ml/min and 254 nm, respectively. the mobile phase was composed from acetonitrile and water, and the gradient elution mode were applied.
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KEYWORD
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Green tea, Catechins, Sovent extraction, HPLC on-line ABTS+
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